Increased levels of hexacosanoic acid in the brain of Wistar rats: a behavioral study

Introduction: X-linked adrenoleukodystrophy (X-ALD) is a peroxisomal metabolic disorder associated with mutations in the ATP-binding cassette sub-family D member1 ( ABCD1 ) gene. Practically all male patients X-ALD develop adrenocortical insufficiency during childhood and progressive myelopathy peripheral neuropathy adulthood. However, some fatal cerebral demyelinating disease named adrenoleukodystrophy. Although exact mechanisms underlying brain damage are still poorly elucidated, it known that hexacosanoic acid (C26:0) accumulation represents hallmark pathogenesis of this disease. In study, we examined whether an overload C26:0 injected Wistar rats was capable causing behavioral changes these animals. Methods: Egg lecithin ethanol dried under nitrogen stream mixed methyl ester. Male at 2–3 weeks age were obtained from Universidade Federal do Rio Grande Sul (UFRGS), divided into 8 groups, submitted to open field test. We then analyzed line crossings (locomotion exploration), rearing (orienting investigatory responses), grooming (anxiety manifestation), latency move for each animal. Results: Animals subjected administration presented fewer episodes higher 45 minutes after injection. The present work yields experimental evidence C26:0, main accumulated metabolite X-ALD, can cause alterations such as impairment locomotion exploratory capabilities, well reduction orienting responses. Conclusion: our results preliminary, they extremely important future studies investigate locomotor X-ALD.